Comparison of tissue culture-induced variation in triticale regenerants obtained by androgenesis and somatic embryogenesis

Abstract Triticale is becoming an increasingly important livestock crop production. This evidenced by increasing triticale-producing areas and improved yields. In addition, meeting the demand for cereals involves introduction of high-yielding stress-resistant varieties into breeding. vitro culture techniques can accelerate development new varieties. Therefore, it seems extremely to develop efficient plant regeneration methods through in cultures understand mechanisms involved gaining regenerants. Obtaining regenerants triticale somatic embryogenesis androgenesis may lead tissue culture-induced variation. present study, we compared obtained both systems (anther immature zygotic embryo cultures), considering level genetic epigenetic changes observed different DNA sequence contexts methylated cytosine (CG, CHG, CHH). The concerning (so-called variation) methylation patterns, i.e., removal (DNA demethylation) (de novo methylation), were analyzed. We that derived via differ notably demethylation symmetrical CG context de asymmetrical CHH context. These be related reprogramming microspore from gametophytic sporophytic lack such process embryos.